pe cy7 conjugated rat igg1 Search Results


anti rat igg f ab 2 apc cy7  (Santa Cruz Biotechnology)
85
Santa Cruz Biotechnology anti rat igg f ab 2 apc cy7
Anti Rat Igg F Ab 2 Apc Cy7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd19 pe-cy7 rat igg2a  (Becton Dickinson)
90
Becton Dickinson cd19 pe-cy7 rat igg2a
Flow Cytometry Antibodies
Cd19 Pe Cy7 Rat Igg2a, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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live/dead bv395 n/a  (Thermo Fisher)
90
Thermo Fisher live/dead bv395 n/a
Flow Cytometry Antibodies
Live/Dead Bv395 N/A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti–cd25-pe-cy7  (Thermo Fisher)
90
Thermo Fisher anti–cd25-pe-cy7
Flow Cytometry Antibodies
Anti–Cd25 Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti-ckit pe-cy7 igg2b  (Becton Dickinson)
90
Becton Dickinson rat anti-ckit pe-cy7 igg2b
Flow Cytometry Antibodies
Rat Anti Ckit Pe Cy7 Igg2b, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-cy7 anti-cd25 (clone m-a251, cat: 560920)  (Becton Dickinson)
90
Becton Dickinson pe-cy7 anti-cd25 (clone m-a251, cat: 560920)
PKA inhibition affects the immunometabolic asset and phenotype of Tconv cells during their diffentiation towards iTreg cells and their suppressive function ( a ) Left, kinetic profile of ECAR in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 12 h. ECAR was measured in real time, under basal conditions and in response to glucose, oligomycin, and 2-DG. Data are shown from three independent experiments at least in technical duplicates (n = 10). Right, parameters of the glycolytic pathway were calculated from the ECAR profile of Tconv cells in the above-mentioned conditions. Data are expressed as mean ± SEM of three different measurements, each of them in ten replicates (n = 30). ( b ) Representative dot plots (left) and cumulative data (right) of <t>CD25,</t> FoxP3-All and FoxP3-E2 in mPKAI-Tconv and CTR-Tconv cells. Data are shown from eleven independent experiments (n = 11). ( c ) Scatter plots showing the expression of CTLA-4, PD-1 and GITR gated on CD4 + FoxP3-All + (top) or CD4 + FoxP3-E2 + (bottom) in mPKAI-Tconv and CTR-Tconv cells. Data are shown as mean ± SEM from four independent experiments in duplicates (n = 8). ( d ) Left, flow cytometry histograms showing proliferation of CFSE + CD4 + T cells TCR-stimulated for 96 h in vitro and cultured alone (empty curves) or in the presence of various numbers of flow-sorted iTreg from mPKAI-Tconv and CTR-Tconv cells. Numbers in plots indicate the percent of CSFE dilution in CD4 + T cells cultured alone (top left) and co-cultured with iTreg cells (above bracketed lines), as indicated. Right, cumulative data of CD4 + T cell proliferation in the above conditions. Data are shown from four independent experiments in duplicates (n = 8). Independet experiments refer to different individuals.
Pe Cy7 Anti Cd25 (Clone M A251, Cat: 560920), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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rat igg2a k isotype control pe-cy7  (Thermo Fisher)
90
Thermo Fisher rat igg2a k isotype control pe-cy7
PKA inhibition affects the immunometabolic asset and phenotype of Tconv cells during their diffentiation towards iTreg cells and their suppressive function ( a ) Left, kinetic profile of ECAR in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 12 h. ECAR was measured in real time, under basal conditions and in response to glucose, oligomycin, and 2-DG. Data are shown from three independent experiments at least in technical duplicates (n = 10). Right, parameters of the glycolytic pathway were calculated from the ECAR profile of Tconv cells in the above-mentioned conditions. Data are expressed as mean ± SEM of three different measurements, each of them in ten replicates (n = 30). ( b ) Representative dot plots (left) and cumulative data (right) of <t>CD25,</t> FoxP3-All and FoxP3-E2 in mPKAI-Tconv and CTR-Tconv cells. Data are shown from eleven independent experiments (n = 11). ( c ) Scatter plots showing the expression of CTLA-4, PD-1 and GITR gated on CD4 + FoxP3-All + (top) or CD4 + FoxP3-E2 + (bottom) in mPKAI-Tconv and CTR-Tconv cells. Data are shown as mean ± SEM from four independent experiments in duplicates (n = 8). ( d ) Left, flow cytometry histograms showing proliferation of CFSE + CD4 + T cells TCR-stimulated for 96 h in vitro and cultured alone (empty curves) or in the presence of various numbers of flow-sorted iTreg from mPKAI-Tconv and CTR-Tconv cells. Numbers in plots indicate the percent of CSFE dilution in CD4 + T cells cultured alone (top left) and co-cultured with iTreg cells (above bracketed lines), as indicated. Right, cumulative data of CD4 + T cell proliferation in the above conditions. Data are shown from four independent experiments in duplicates (n = 8). Independet experiments refer to different individuals.
Rat Igg2a K Isotype Control Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat igg 2b -pe-cy7 (eb149/10h5)  (Thermo Fisher)
90
Thermo Fisher rat igg 2b -pe-cy7 (eb149/10h5)
PKA inhibition affects the immunometabolic asset and phenotype of Tconv cells during their diffentiation towards iTreg cells and their suppressive function ( a ) Left, kinetic profile of ECAR in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 12 h. ECAR was measured in real time, under basal conditions and in response to glucose, oligomycin, and 2-DG. Data are shown from three independent experiments at least in technical duplicates (n = 10). Right, parameters of the glycolytic pathway were calculated from the ECAR profile of Tconv cells in the above-mentioned conditions. Data are expressed as mean ± SEM of three different measurements, each of them in ten replicates (n = 30). ( b ) Representative dot plots (left) and cumulative data (right) of <t>CD25,</t> FoxP3-All and FoxP3-E2 in mPKAI-Tconv and CTR-Tconv cells. Data are shown from eleven independent experiments (n = 11). ( c ) Scatter plots showing the expression of CTLA-4, PD-1 and GITR gated on CD4 + FoxP3-All + (top) or CD4 + FoxP3-E2 + (bottom) in mPKAI-Tconv and CTR-Tconv cells. Data are shown as mean ± SEM from four independent experiments in duplicates (n = 8). ( d ) Left, flow cytometry histograms showing proliferation of CFSE + CD4 + T cells TCR-stimulated for 96 h in vitro and cultured alone (empty curves) or in the presence of various numbers of flow-sorted iTreg from mPKAI-Tconv and CTR-Tconv cells. Numbers in plots indicate the percent of CSFE dilution in CD4 + T cells cultured alone (top left) and co-cultured with iTreg cells (above bracketed lines), as indicated. Right, cumulative data of CD4 + T cell proliferation in the above conditions. Data are shown from four independent experiments in duplicates (n = 8). Independet experiments refer to different individuals.
Rat Igg 2b Pe Cy7 (Eb149/10h5), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-cd25-percp-efluor710/apc cy7 4e3-ebioscience/clone m-a251  (Thermo Fisher)
90
Thermo Fisher anti-cd25-percp-efluor710/apc cy7 4e3-ebioscience/clone m-a251
PKA inhibition affects the immunometabolic asset and phenotype of Tconv cells during their diffentiation towards iTreg cells and their suppressive function ( a ) Left, kinetic profile of ECAR in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 12 h. ECAR was measured in real time, under basal conditions and in response to glucose, oligomycin, and 2-DG. Data are shown from three independent experiments at least in technical duplicates (n = 10). Right, parameters of the glycolytic pathway were calculated from the ECAR profile of Tconv cells in the above-mentioned conditions. Data are expressed as mean ± SEM of three different measurements, each of them in ten replicates (n = 30). ( b ) Representative dot plots (left) and cumulative data (right) of <t>CD25,</t> FoxP3-All and FoxP3-E2 in mPKAI-Tconv and CTR-Tconv cells. Data are shown from eleven independent experiments (n = 11). ( c ) Scatter plots showing the expression of CTLA-4, PD-1 and GITR gated on CD4 + FoxP3-All + (top) or CD4 + FoxP3-E2 + (bottom) in mPKAI-Tconv and CTR-Tconv cells. Data are shown as mean ± SEM from four independent experiments in duplicates (n = 8). ( d ) Left, flow cytometry histograms showing proliferation of CFSE + CD4 + T cells TCR-stimulated for 96 h in vitro and cultured alone (empty curves) or in the presence of various numbers of flow-sorted iTreg from mPKAI-Tconv and CTR-Tconv cells. Numbers in plots indicate the percent of CSFE dilution in CD4 + T cells cultured alone (top left) and co-cultured with iTreg cells (above bracketed lines), as indicated. Right, cumulative data of CD4 + T cell proliferation in the above conditions. Data are shown from four independent experiments in duplicates (n = 8). Independet experiments refer to different individuals.
Anti Cd25 Percp Efluor710/Apc Cy7 4e3 Ebioscience/Clone M A251, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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anti-rat igg2a κ isotype control pe-cy7 (ebr2a  (Thermo Fisher)
90
Thermo Fisher anti-rat igg2a κ isotype control pe-cy7 (ebr2a
PKA inhibition affects the immunometabolic asset and phenotype of Tconv cells during their diffentiation towards iTreg cells and their suppressive function ( a ) Left, kinetic profile of ECAR in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 12 h. ECAR was measured in real time, under basal conditions and in response to glucose, oligomycin, and 2-DG. Data are shown from three independent experiments at least in technical duplicates (n = 10). Right, parameters of the glycolytic pathway were calculated from the ECAR profile of Tconv cells in the above-mentioned conditions. Data are expressed as mean ± SEM of three different measurements, each of them in ten replicates (n = 30). ( b ) Representative dot plots (left) and cumulative data (right) of <t>CD25,</t> FoxP3-All and FoxP3-E2 in mPKAI-Tconv and CTR-Tconv cells. Data are shown from eleven independent experiments (n = 11). ( c ) Scatter plots showing the expression of CTLA-4, PD-1 and GITR gated on CD4 + FoxP3-All + (top) or CD4 + FoxP3-E2 + (bottom) in mPKAI-Tconv and CTR-Tconv cells. Data are shown as mean ± SEM from four independent experiments in duplicates (n = 8). ( d ) Left, flow cytometry histograms showing proliferation of CFSE + CD4 + T cells TCR-stimulated for 96 h in vitro and cultured alone (empty curves) or in the presence of various numbers of flow-sorted iTreg from mPKAI-Tconv and CTR-Tconv cells. Numbers in plots indicate the percent of CSFE dilution in CD4 + T cells cultured alone (top left) and co-cultured with iTreg cells (above bracketed lines), as indicated. Right, cumulative data of CD4 + T cell proliferation in the above conditions. Data are shown from four independent experiments in duplicates (n = 8). Independet experiments refer to different individuals.
Anti Rat Igg2a κ Isotype Control Pe Cy7 (Ebr2a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secondary antibodies  (SouthernBiotech)
93
SouthernBiotech secondary antibodies
PKA inhibition affects the immunometabolic asset and phenotype of Tconv cells during their diffentiation towards iTreg cells and their suppressive function ( a ) Left, kinetic profile of ECAR in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 12 h. ECAR was measured in real time, under basal conditions and in response to glucose, oligomycin, and 2-DG. Data are shown from three independent experiments at least in technical duplicates (n = 10). Right, parameters of the glycolytic pathway were calculated from the ECAR profile of Tconv cells in the above-mentioned conditions. Data are expressed as mean ± SEM of three different measurements, each of them in ten replicates (n = 30). ( b ) Representative dot plots (left) and cumulative data (right) of <t>CD25,</t> FoxP3-All and FoxP3-E2 in mPKAI-Tconv and CTR-Tconv cells. Data are shown from eleven independent experiments (n = 11). ( c ) Scatter plots showing the expression of CTLA-4, PD-1 and GITR gated on CD4 + FoxP3-All + (top) or CD4 + FoxP3-E2 + (bottom) in mPKAI-Tconv and CTR-Tconv cells. Data are shown as mean ± SEM from four independent experiments in duplicates (n = 8). ( d ) Left, flow cytometry histograms showing proliferation of CFSE + CD4 + T cells TCR-stimulated for 96 h in vitro and cultured alone (empty curves) or in the presence of various numbers of flow-sorted iTreg from mPKAI-Tconv and CTR-Tconv cells. Numbers in plots indicate the percent of CSFE dilution in CD4 + T cells cultured alone (top left) and co-cultured with iTreg cells (above bracketed lines), as indicated. Right, cumulative data of CD4 + T cell proliferation in the above conditions. Data are shown from four independent experiments in duplicates (n = 8). Independet experiments refer to different individuals.
Secondary Antibodies, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
secondary antibodies - by Bioz Stars, 2026-03
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pe-cy7-conjugated rat immunoglobulin g1 (igg1)  (Thermo Fisher)
90
Thermo Fisher pe-cy7-conjugated rat immunoglobulin g1 (igg1)
PKA inhibition affects the immunometabolic asset and phenotype of Tconv cells during their diffentiation towards iTreg cells and their suppressive function ( a ) Left, kinetic profile of ECAR in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 12 h. ECAR was measured in real time, under basal conditions and in response to glucose, oligomycin, and 2-DG. Data are shown from three independent experiments at least in technical duplicates (n = 10). Right, parameters of the glycolytic pathway were calculated from the ECAR profile of Tconv cells in the above-mentioned conditions. Data are expressed as mean ± SEM of three different measurements, each of them in ten replicates (n = 30). ( b ) Representative dot plots (left) and cumulative data (right) of <t>CD25,</t> FoxP3-All and FoxP3-E2 in mPKAI-Tconv and CTR-Tconv cells. Data are shown from eleven independent experiments (n = 11). ( c ) Scatter plots showing the expression of CTLA-4, PD-1 and GITR gated on CD4 + FoxP3-All + (top) or CD4 + FoxP3-E2 + (bottom) in mPKAI-Tconv and CTR-Tconv cells. Data are shown as mean ± SEM from four independent experiments in duplicates (n = 8). ( d ) Left, flow cytometry histograms showing proliferation of CFSE + CD4 + T cells TCR-stimulated for 96 h in vitro and cultured alone (empty curves) or in the presence of various numbers of flow-sorted iTreg from mPKAI-Tconv and CTR-Tconv cells. Numbers in plots indicate the percent of CSFE dilution in CD4 + T cells cultured alone (top left) and co-cultured with iTreg cells (above bracketed lines), as indicated. Right, cumulative data of CD4 + T cell proliferation in the above conditions. Data are shown from four independent experiments in duplicates (n = 8). Independet experiments refer to different individuals.
Pe Cy7 Conjugated Rat Immunoglobulin G1 (Igg1), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Flow Cytometry Antibodies

Journal: Endocrinology

Article Title: The Thyroid Hormone Inactivating Type 3 Deiodinase Is Essential for Optimal Neutrophil Function: Observations From Three Species

doi: 10.1210/en.2017-00666

Figure Lengend Snippet: Flow Cytometry Antibodies

Article Snippet: Manufacturer Ly-6G PerCP-Cy5.5 Rat IgG2A; monoclonal 1A8 560602 BD Biosciences Ly-6C PE Rat IgG2c; monoclonal HK1.4 12-5932 eBioscience CD117 (cKit) APC Rat IgG2b; monoclonal 2B8 17-1171 eBioscience CD11b APC-Cy7 Rat IgG2b; monoclonal M1/70 557657 BD Biosciences CD19 PE-Cy7 Rat IgG2A; monoclonal 1D3 25-0193 eBioscience CD335 PE-Cy7 Rat IgG2A; monoclonal 29A1.4 25-3351 eBioscience CD3e PE-Cy7 Armenian hamster; monoclonal 145-2C11 25-0031 eBioscience CD16/CD32 (FC block) Rat IgG2A; monoclonal 93 14-0161 eBioscience Open in a separate window Flow Cytometry Antibodies Analysis of neutrophil counts in whole blood After euthanasia, blood was extracted from the cava vein of D3KO and WT mice, and 40 µL of each sample was collected in EDTA microtainer tubes.

Techniques: Flow Cytometry, Blocking Assay

Antibody Table

Journal: Endocrinology

Article Title: The Thyroid Hormone Inactivating Type 3 Deiodinase Is Essential for Optimal Neutrophil Function: Observations From Three Species

doi: 10.1210/en.2017-00666

Figure Lengend Snippet: Antibody Table

Article Snippet: Manufacturer Ly-6G PerCP-Cy5.5 Rat IgG2A; monoclonal 1A8 560602 BD Biosciences Ly-6C PE Rat IgG2c; monoclonal HK1.4 12-5932 eBioscience CD117 (cKit) APC Rat IgG2b; monoclonal 2B8 17-1171 eBioscience CD11b APC-Cy7 Rat IgG2b; monoclonal M1/70 557657 BD Biosciences CD19 PE-Cy7 Rat IgG2A; monoclonal 1D3 25-0193 eBioscience CD335 PE-Cy7 Rat IgG2A; monoclonal 29A1.4 25-3351 eBioscience CD3e PE-Cy7 Armenian hamster; monoclonal 145-2C11 25-0031 eBioscience CD16/CD32 (FC block) Rat IgG2A; monoclonal 93 14-0161 eBioscience Open in a separate window Flow Cytometry Antibodies Analysis of neutrophil counts in whole blood After euthanasia, blood was extracted from the cava vein of D3KO and WT mice, and 40 µL of each sample was collected in EDTA microtainer tubes.

Techniques: Sequencing, Blocking Assay

PKA inhibition affects the immunometabolic asset and phenotype of Tconv cells during their diffentiation towards iTreg cells and their suppressive function ( a ) Left, kinetic profile of ECAR in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 12 h. ECAR was measured in real time, under basal conditions and in response to glucose, oligomycin, and 2-DG. Data are shown from three independent experiments at least in technical duplicates (n = 10). Right, parameters of the glycolytic pathway were calculated from the ECAR profile of Tconv cells in the above-mentioned conditions. Data are expressed as mean ± SEM of three different measurements, each of them in ten replicates (n = 30). ( b ) Representative dot plots (left) and cumulative data (right) of CD25, FoxP3-All and FoxP3-E2 in mPKAI-Tconv and CTR-Tconv cells. Data are shown from eleven independent experiments (n = 11). ( c ) Scatter plots showing the expression of CTLA-4, PD-1 and GITR gated on CD4 + FoxP3-All + (top) or CD4 + FoxP3-E2 + (bottom) in mPKAI-Tconv and CTR-Tconv cells. Data are shown as mean ± SEM from four independent experiments in duplicates (n = 8). ( d ) Left, flow cytometry histograms showing proliferation of CFSE + CD4 + T cells TCR-stimulated for 96 h in vitro and cultured alone (empty curves) or in the presence of various numbers of flow-sorted iTreg from mPKAI-Tconv and CTR-Tconv cells. Numbers in plots indicate the percent of CSFE dilution in CD4 + T cells cultured alone (top left) and co-cultured with iTreg cells (above bracketed lines), as indicated. Right, cumulative data of CD4 + T cell proliferation in the above conditions. Data are shown from four independent experiments in duplicates (n = 8). Independet experiments refer to different individuals.

Journal: Scientific Reports

Article Title: Deciphering the role of protein kinase A in the control of FoxP3 expression in regulatory T cells in health and autoimmunity

doi: 10.1038/s41598-024-68098-z

Figure Lengend Snippet: PKA inhibition affects the immunometabolic asset and phenotype of Tconv cells during their diffentiation towards iTreg cells and their suppressive function ( a ) Left, kinetic profile of ECAR in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 12 h. ECAR was measured in real time, under basal conditions and in response to glucose, oligomycin, and 2-DG. Data are shown from three independent experiments at least in technical duplicates (n = 10). Right, parameters of the glycolytic pathway were calculated from the ECAR profile of Tconv cells in the above-mentioned conditions. Data are expressed as mean ± SEM of three different measurements, each of them in ten replicates (n = 30). ( b ) Representative dot plots (left) and cumulative data (right) of CD25, FoxP3-All and FoxP3-E2 in mPKAI-Tconv and CTR-Tconv cells. Data are shown from eleven independent experiments (n = 11). ( c ) Scatter plots showing the expression of CTLA-4, PD-1 and GITR gated on CD4 + FoxP3-All + (top) or CD4 + FoxP3-E2 + (bottom) in mPKAI-Tconv and CTR-Tconv cells. Data are shown as mean ± SEM from four independent experiments in duplicates (n = 8). ( d ) Left, flow cytometry histograms showing proliferation of CFSE + CD4 + T cells TCR-stimulated for 96 h in vitro and cultured alone (empty curves) or in the presence of various numbers of flow-sorted iTreg from mPKAI-Tconv and CTR-Tconv cells. Numbers in plots indicate the percent of CSFE dilution in CD4 + T cells cultured alone (top left) and co-cultured with iTreg cells (above bracketed lines), as indicated. Right, cumulative data of CD4 + T cell proliferation in the above conditions. Data are shown from four independent experiments in duplicates (n = 8). Independet experiments refer to different individuals.

Article Snippet: For the simultaneous evaluation of surface and intracellular molecules, human iTreg cells were stained with the following antibodies: FITC anti-CD4 (Clone RPA-T4, Cat: 561842) (BD Pharmingen), PE-Cy7 anti-CD25 (Clone M-A251, Cat: 560920) (cBD Pharmingen), APC anti-CD152/CTLA-4 (Clone BNI3, Cat: 555855) (BD Pharmingen), PE-Cy5 anti-GITR (Clone REA1007, Cat: 130-116-842) (Miltenyi Biotec), BV421 anti-CD279/PD-1 (Clone EH12.1, Cat: 562516) (BD Horizon), PE anti-FoxP3-All (Clone PCH101, Cat: 560046) (eBioscience) and PE anti-FoxP3-E2 (Clone 150D/E4, Cat: 12-4774-42) (BD Pharmingen).

Techniques: Inhibition, Expressing, Flow Cytometry, In Vitro, Cell Culture